Structure and immunomodulatory activity of <i>Lentinus edodes</i> polysaccharides modified by probiotic fermentation
نویسندگان
چکیده
Plant-based fermentations provide an untapped source for novel biotechnological applications. In this study, a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes. Polysaccharides were extracted from fermented and non-fermented L. edodes purified via DEAE-52 Sephadex G-100. The components designated F-LEP-2a NF-LEP-2a analyzed by FT-IR, HPGPC, HPAEC, SEM, GC-MS NMR. results revealed that fermentation increased the molecular weight 1.16 × 104 Da 1.87 altered proportions of glucose, galactose mannose, in which glucose 45.94% 48.16%. Methylation analysis NMR spectra indicated had similar linkage patterns. Furthermore, their immunomodulatory activities evaluated with immunosuppressive mice. NF-LEP F-LEP improved immune organ indices, immunoglobulin (IgG IgM) cytokines concentrations; restored antioxidation capacity liver; maintained balance gut microbiota. displayed better moderating effects on spleen index, immunoglobulin, diversity microbiota than (200, 400 mg/kg). Our study provides efficient environment-friendly way structural modification polysaccharides, helps enhance biological activity promote wide application food, medicine other fields.
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ژورنال
عنوان ژورنال: Food Science and Human Wellness
سال: 2024
ISSN: ['2213-4530']
DOI: https://doi.org/10.26599/fshw.2022.9250036